14-3-3 sigma Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52005r-cy5-100uL |
| Product Name | 14-3-3 sigma Monoclonal Antibody, Cy5 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Recombinant human 14-3-3 sigma protein, full length. |
| Clonality | Monoclonal |
| Clone # | 2E12 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2810 |
| Swiss Prot | P31947 |
| Synonyms | 14-3-3 protein sigma; SFN; Epithelial cell marker protein 1; Stratifin; HME1 |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication p53-regulated inhibitor of G2/M progression. |
| Application Dilution | |
| IF | 1:50-200 |
Frequently asked questions
Which applications is 14-3-3 sigma Monoclonal Antibody, Cy5 Conjugated (bsm-52005R-Cy5) validated for, and at what dilution?
bsm-52005R-Cy5 is validated for IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 14-3-3 sigma Monoclonal Antibody, Cy5 Conjugated (bsm-52005R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52005R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 14-3-3 sigma Monoclonal Antibody, Cy5 Conjugated (bsm-52005R-Cy5)?
Recombinant human 14-3-3 sigma protein, full length.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52005R-Cy5)?
Cy5 on bsm-52005R-Cy5: excitation / emission 625,650nm/670nm.