ARF1 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52016r-cy5-100uL |
| Product Name | ARF1 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Recombinant human ARF1 protein, full length. |
| Clonality | Monoclonal |
| Clone # | 1D4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 375 |
| Swiss Prot | P84077 |
| Synonyms | ADP-ribosylation factor 1; ARF1 |
| Background | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is ARF1 Recombinant Antibody, Cy5 Conjugated (bsm-52016R-Cy5) validated for, and at what dilution?
bsm-52016R-Cy5 is validated for WB (1:300-5000); IF (1:50-200); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ARF1 Recombinant Antibody, Cy5 Conjugated (bsm-52016R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52016R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ARF1 Recombinant Antibody, Cy5 Conjugated (bsm-52016R-Cy5)?
Recombinant human ARF1 protein, full length.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52016R-Cy5)?
Cy5 on bsm-52016R-Cy5: excitation / emission 625,650nm/670nm.