AKT1(T450) Recombinant Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52131r-percp-cy7-100uL |
| Product Name | AKT1(T450) Recombinant Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | Synthetic peptide derived from human AKT1(T450), around 400-470aa (phospho T450). |
| Modification Site | T450 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 207 |
| Swiss Prot | P31749 |
| Synonyms | AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; PKBG |
| Background | AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is AKT1(T450) Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52131R-PerCP-Cy7) validated for, and at what dilution?
bsm-52131R-PerCP-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AKT1(T450) Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52131R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52131R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AKT1(T450) Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52131R-PerCP-Cy7)?
Synthetic peptide derived from human AKT1(T450), around 400-470aa (phospho T450).
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bsm-52131R-PerCP-Cy7)?
PerCP-Cy7 on bsm-52131R-PerCP-Cy7: excitation / emission 482nm/780nm.