PIK3R1 Monoclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52219m-percp-cy55-100ul |
| Product Name | PIK3R1 Monoclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Mouse |
| Source | Recombinant human PI3-kinase p85 subunit alpha protein, around 600-700aa. |
| Clonality | Monoclonal |
| Clone # | 9B3 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5295 |
| Swiss Prot | P27986 |
| Synonyms | Phosphatidylinositol 3-kinase regulatory subunit alpha; PIK3R1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PIK3R1; GRB1; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha |
| Background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is PIK3R1 Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-52219M-PERCP-CY5-5) validated for, and at what dilution?
BSM-52219M-PERCP-CY5-5 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PIK3R1 Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-52219M-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52219M-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PIK3R1 Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-52219M-PERCP-CY5-5)?
Recombinant human PI3-kinase p85 subunit alpha protein, around 600-700aa.
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-52219M-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-52219M-PERCP-CY5-5: excitation / emission 482nm/694nm.