YY1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52349r-cy3-100uL |
| Product Name | YY1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant human YY1 between 250 to 350 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7528 |
| Swiss Prot | P25490 |
| Synonyms | Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1 |
| Background | YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. |
| Application Dilution | |
| WB | 1:100-200 |
| IF | 1:200-800 |
| Flow-Cyt | 1:500-2000 |
Frequently asked questions
Which applications is YY1 Recombinant Antibody, Cy3 Conjugated (bsm-52349R-Cy3) validated for, and at what dilution?
bsm-52349R-Cy3 is validated for WB (1:100-200); IF (1:200-800); Flow-Cyt (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does YY1 Recombinant Antibody, Cy3 Conjugated (bsm-52349R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52349R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise YY1 Recombinant Antibody, Cy3 Conjugated (bsm-52349R-Cy3)?
Recombinant human YY1 between 250 to 350 amino acids
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-52349R-Cy3)?
Cy3 on bsm-52349R-Cy3: excitation / emission 512,550nm/570,615nm.