alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52396r-bf350-100uL |
| Product Name | alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Actin alpha |
| Clonality | Recombinant |
| Clone # | 2B10 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59 |
| Swiss Prot | P62736 |
| Subcellular location | Cytoplasm |
| Synonyms | ACTSA; SMDYS; 0610041G09Rik; Actvs; SMAalpha; SMalphaA; a-SMA; alphaSMA; ACT-4; actin; ACTA_BOVIN; ACTA2; Alpha-actin-2; 3.6.4.-; ACTA_HUMAN; Cell growth-inhibiting gene 46 protein; ACTA_MOUSE; ACTA_RABIT; ACTA_RAT; actin alpha 2, smooth muscle; actin, alpha 2, smooth muscle, aorta |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Application Dilution | |
| WB | 1:1000-5000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:100-200 |
Frequently asked questions
Which applications is alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52396R-BF350) validated for, and at what dilution?
bsm-52396R-BF350 is validated for WB (1:1000-5000); IF (1:100-500); Flow-Cyt (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52396R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Zebrafish.
How should bsm-52396R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha smooth muscle Actin Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52396R-BF350)?
KLH conjugated synthetic peptide derived from human Actin alpha
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-52396R-BF350)?
AbBy Fluor-350 on bsm-52396R-BF350: excitation / emission 346nm/442nm.