Topoisomerase I Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52531r-biotin-100uL |
| Product Name | Topoisomerase I Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Human Topoisomerase I between 1-100 amino acids |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7150 |
| Swiss Prot | P11387 |
| Synonyms | DNA topoisomerase 1, DNA topoisomerase I, TOP1 |
| Background | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:50-200 |
| IHC-F | IHC-F1:50-200 |
Frequently asked questions
Which applications is Topoisomerase I Recombinant Antibody, Biotin Conjugated (bsm-52531R-Biotin) validated for, and at what dilution?
bsm-52531R-Biotin is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Topoisomerase I Recombinant Antibody, Biotin Conjugated (bsm-52531R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52531R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Topoisomerase I Recombinant Antibody, Biotin Conjugated (bsm-52531R-Biotin)?
Human Topoisomerase I between 1-100 amino acids