Tyrosine Hydroxylase Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52574r-Cy5.5-100uL |
| Product Name | Tyrosine Hydroxylase Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Human Tyrosine Hydroxylase between 500 amino acid to C-terminus |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7054 |
| Swiss Prot | P07101 |
| Synonyms | Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH; TYH |
| Background | Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease. |
| Application Dilution | |
| WB | IFICC1:50-200 |
| FCM | WB1:500-2000 |
| IF(IHC-P) | IHC-P1:100-500 |
| IF(ICC) | FCM1:50-100 |
Frequently asked questions
Which applications is Tyrosine Hydroxylase Recombinant Antibody, Cy5.5 Conjugated (BSM-52574R-CY5-5) validated for, and at what dilution?
BSM-52574R-CY5-5 is validated for WB(IF(ICC)) (1:50-200); FCM(WB) (1:500-2000); IF(IHC-P)(IHC-P) (1:100-500); IF(ICC)(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tyrosine Hydroxylase Recombinant Antibody, Cy5.5 Conjugated (BSM-52574R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52574R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tyrosine Hydroxylase Recombinant Antibody, Cy5.5 Conjugated (BSM-52574R-CY5-5)?
Human Tyrosine Hydroxylase between 500 amino acid to C-terminus
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-52574R-CY5-5)?
Cy5.5 on BSM-52574R-CY5-5: excitation / emission 675nm/694nm.