RUNX2 Recombinant Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52672r-percp-cy7-100uL |
| Product Name | RUNX2 Recombinant Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | Human RUNX2 between 300 to 450 amino acids |
| Immunogen Range | 300-450/521 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 860 |
| Swiss Prot | Q13950 |
| Synonyms | Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2 |
| Background | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is RUNX2 Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52672R-PerCP-Cy7) validated for, and at what dilution?
bsm-52672R-PerCP-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RUNX2 Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52672R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52672R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RUNX2 Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-52672R-PerCP-Cy7)?
Human RUNX2 between 300 to 450 amino acids Immunogen range: 300-450/521.
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bsm-52672R-PerCP-Cy7)?
PerCP-Cy7 on bsm-52672R-PerCP-Cy7: excitation / emission 482nm/780nm.