TSG101 Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52746r-cy55-100ul |
| Product Name | TSG101 Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Human TSG101 between 1 to 100 amino acids |
| Immunogen Range | 30-70/390 |
| Clonality | Monoclonal |
| Clone # | 6C3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7251 |
| Swiss Prot | Q99816 |
| Synonyms | Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101 |
| Background | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is TSG101 Recombinant Antibody, Cy5.5 Conjugated (BSM-52746R-CY5-5) validated for, and at what dilution?
BSM-52746R-CY5-5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSG101 Recombinant Antibody, Cy5.5 Conjugated (BSM-52746R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52746R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSG101 Recombinant Antibody, Cy5.5 Conjugated (BSM-52746R-CY5-5)?
Human TSG101 between 1 to 100 amino acids Immunogen range: 30-70/390.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-52746R-CY5-5)?
Cy5.5 on BSM-52746R-CY5-5: excitation / emission 675nm/694nm.