CLOCK Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54050r-rbitc-100uL |
| Product Name | CLOCK Recombinant Antibody, RBITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant protein |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9575 |
| Swiss Prot | O15516 |
| Synonyms | Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, hCLOCK, bHLHe8, CLOCK, BHLHE8, KIAA0334; clock circadian regulator |
| Background | Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the Basic-helix-loop-helix-psp (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the clock gene have been shown to increase the length of the endogenous period and To contain a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation ability. It has been speculated that Clock may regulation circadian rhythmicity in combination with Other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CLOCK Recombinant Antibody, RBITC Conjugated (bsm-54050R-RBITC) validated for, and at what dilution?
bsm-54050R-RBITC is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CLOCK Recombinant Antibody, RBITC Conjugated (bsm-54050R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54050R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CLOCK Recombinant Antibody, RBITC Conjugated (bsm-54050R-RBITC)?
Recombinant protein
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54050R-RBITC)?
RBITC on bsm-54050R-RBITC: excitation / emission 570nm/595nm.