alpha Internexin Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54173r-cy55-100uL |
| Product Name | alpha Internexin Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Recombinant protein within human alpha Internexin aa 350-499 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9118 |
| Swiss Prot | Q16352 |
| Synonyms | Alpha-internexin; 66 kDa neurofilament protein; Neurofilament 5; Alpha-Inx; NF-66; Neurofilament-66; INA; NEF5; internexin neuronal intermediate filament protein alpha. |
| Background | Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
| IF | |
Frequently asked questions
Which applications is alpha Internexin Recombinant Antibody, Cy5.5 Conjugated (BSM-54173R-CY5-5) validated for, and at what dilution?
BSM-54173R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha Internexin Recombinant Antibody, Cy5.5 Conjugated (BSM-54173R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-54173R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha Internexin Recombinant Antibody, Cy5.5 Conjugated (BSM-54173R-CY5-5)?
Recombinant protein within human alpha Internexin aa 350-499
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-54173R-CY5-5)?
Cy5.5 on BSM-54173R-CY5-5: excitation / emission 675nm/694nm.