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ZAP70 Recombinant Antibody, RBITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-54192r-rbitc-100uL
Product Name ZAP70 Recombinant Antibody, RBITC Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source A synthesized peptide derived from human ZAP70
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7535
Swiss Prot P43403
Subcellular location Cytoplasm, Cell membrane
Synonyms Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ZAP70 Recombinant Antibody, RBITC Conjugated (bsm-54192R-RBITC) validated for, and at what dilution?

bsm-54192R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZAP70 Recombinant Antibody, RBITC Conjugated (bsm-54192R-RBITC) react with?

Tested reactivity: Human.

How should bsm-54192R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZAP70 Recombinant Antibody, RBITC Conjugated (bsm-54192R-RBITC)?

A synthesized peptide derived from human ZAP70

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-54192R-RBITC)?

RBITC on bsm-54192R-RBITC: excitation / emission 570nm/595nm.

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