Alpha Synuclein Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54277r-cy3-100uL |
| Product Name | Alpha Synuclein Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant full length Human protein |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6622 |
| Swiss Prot | P37840 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; NACP; PARK1; PARK4 |
| Background | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:200-800 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Alpha Synuclein Recombinant Antibody, Cy3 Conjugated (bsm-54277R-Cy3) validated for, and at what dilution?
bsm-54277R-Cy3 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Alpha Synuclein Recombinant Antibody, Cy3 Conjugated (bsm-54277R-Cy3) react with?
Tested reactivity: Human, Rat.
How should bsm-54277R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Alpha Synuclein Recombinant Antibody, Cy3 Conjugated (bsm-54277R-Cy3)?
Recombinant full length Human protein
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54277R-Cy3)?
Cy3 on bsm-54277R-Cy3: excitation / emission 512,550nm/570,615nm.