H4C1 | Histone H4 (ac-K16) Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54330r-bf350-100uL |
| Product Name | H4C1 | Histone H4 (ac-K16) Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | Recombinant protein |
| Modification Site | K16 |
| Clonality | Recombinant |
| Clone # | 2G2 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121504 |
| Swiss Prot | P62805 |
| Subcellular location | Nucleus |
| Synonyms | H4-16; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; HIST4H4; H4/o; H4C16; HIST2H4B; H4/m; H4FM; H4M; HIST1H4I; TEBIVANED4; TEVANED4; H4FA; HIST1H4A; H4/b; H4FB; HIST1H4D; dJ221C16.9; H4; H4/c; H4FC; HIST1H4F; H4/d; H4F2iii; H4FD; HIST1H4K; dJ160A22.1; H4/e; H4F2iv; H4FE; HIST1H4J; TEBIVANED2; TEVANED2; dJ160A22.2; H4/g; H4FG; HIST1H4C; TEBIVANED1; TEVANED1; dJ221C16.1; H4/h; H4FH; HIST1H4H; H4/I; H4FI; HIST1H4B; H4/j; H4FJ; HIST1H4E; TEBIVANED3; TEVANED3; H4.k; H4/k; H4FK; HIST1H4L; FO108; H4/n; H4F2; H4FN; HIST2H4; HIST2H4A; Gm11275; H4f16; Hist1h4m; Hist1h4n; B130044J01Rik; 1700024H08Rik; H2c8; H4_DROME; His4; His4r; His4:CG31611; His4:CG33869; His4:CG33871; His4:CG33873; His4:CG33875; His4:CG33877; His4:CG33879; His4:CG33881; His4:CG33883; His4:CG33885; His4:CG33887; His4:CG33889; His4:CG33891; His4:CG33893; His4:CG33895; His4:CG33897; His4:CG33899; His4:CG33901; His4:CG33903; His4:CG33905; His4:CG33907; His4:CG33909; H4r; H4_HUMAN; H4/A; H4FO; H4_MOUSE; H4-53; H4-12; H4_SCHPO; hhf1; hhf2; hhf3; h4.1; h4.2; h4.3; H4 clustered histone 1; H4 histone family, member A; histone 1, H4a; histone cluster 1, H4a; histone cluster 1 H4 family member a; H4C1 | Histone H4 (acetyl-K16); H4K16Ac; ac-Histone H4; acetyl-Histone H4; ac-H4; H4C1 | Histone H4 (acetyl-Lys16) |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is H4C1 | Histone H4 (ac-K16) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-54330R-BF350) validated for, and at what dilution?
bsm-54330R-BF350 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H4C1 | Histone H4 (ac-K16) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-54330R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54330R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H4C1 | Histone H4 (ac-K16) Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-54330R-BF350)?
Recombinant protein
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-54330R-BF350)?
AbBy Fluor-350 on bsm-54330R-BF350: excitation / emission 346nm/442nm.