TYROBP Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54408r-cy55-100ul |
| Product Name | TYROBP Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Synthetic peptide within human DAP12 aa 60-100. |
| Clonality | Monoclonal |
| Clone # | 2F4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7305 |
| Swiss Prot | O43914 |
| Subcellular location | Cell membrane |
| Synonyms | TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein; TYROBP; DAP12; KARAP |
| Background | TYROBP is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. TYROBP Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. |
| Application Dilution | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is TYROBP Recombinant Antibody, Cy5.5 Conjugated (BSM-54408R-CY5-5) validated for, and at what dilution?
BSM-54408R-CY5-5 is validated for Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TYROBP Recombinant Antibody, Cy5.5 Conjugated (BSM-54408R-CY5-5) react with?
Tested reactivity: Human.
How should BSM-54408R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TYROBP Recombinant Antibody, Cy5.5 Conjugated (BSM-54408R-CY5-5)?
Synthetic peptide within human DAP12 aa 60-100.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-54408R-CY5-5)?
Cy5.5 on BSM-54408R-CY5-5: excitation / emission 675nm/694nm.