Human Ig Kappa light chain Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54763r-bf405-100uL |
| Product Name | Human Ig Kappa light chain Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | Full length native human IgG. |
| Immunogen Range | 1-107/107 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3514 |
| Swiss Prot | P01834 |
| Subcellular location | Extracellular, Secreted |
| Synonyms | Kappa Light Chain; IGKC; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; Human Ig/L-Chain Kappa; IGKC_HUMAN. |
| Background | Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Human Ig Kappa light chain Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-54763R-BF405) validated for, and at what dilution?
bsm-54763R-BF405 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Human Ig Kappa light chain Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-54763R-BF405) react with?
Tested reactivity: Human.
How should bsm-54763R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Human Ig Kappa light chain Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-54763R-BF405)?
Full length native human IgG. Immunogen range: 1-107/107.
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-54763R-BF405)?
AbBy Fluor-405 on bsm-54763R-BF405: excitation / emission 401nm/421nm.