TOP2A Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61077r-Cy5.5-100uL |
| Product Name | TOP2A Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TOPO II alpha |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7153 |
| Swiss Prot | P11388 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DNA topoisomerasealpha; TOPO II; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN; Topo II; Topo-II; Topoisomerase II-. |
| Background | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is TOP2A Recombinant Antibody, Cy5.5 Conjugated (BSM-61077R-CY5-5) validated for, and at what dilution?
BSM-61077R-CY5-5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TOP2A Recombinant Antibody, Cy5.5 Conjugated (BSM-61077R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61077R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TOP2A Recombinant Antibody, Cy5.5 Conjugated (BSM-61077R-CY5-5)?
KLH conjugated synthetic peptide derived from human TOPO II alpha
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61077R-CY5-5)?
Cy5.5 on BSM-61077R-CY5-5: excitation / emission 675nm/694nm.