APAF1 Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61152r-Cy5.5-100uL |
| Product Name | APAF1 Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APAF 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 317 |
| Swiss Prot | O14727 |
| Subcellular location | Cytoplasm |
| Synonyms | APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413. |
| Background | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is APAF1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61152R-CY5-5) validated for, and at what dilution?
BSM-61152R-CY5-5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APAF1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61152R-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BSM-61152R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APAF1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61152R-CY5-5)?
KLH conjugated synthetic peptide derived from human APAF 1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61152R-CY5-5)?
Cy5.5 on BSM-61152R-CY5-5: excitation / emission 675nm/694nm.