Phospho-Histone H2B.1 (Thr129) Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61195r-Cy5.5-100uL |
| Product Name | Phospho-Histone H2B.1 (Thr129) Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Yeast |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129 |
| Modification Site | Thr129 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 851810 |
| Swiss Prot | P02293 |
| Synonyms | HTB2; Htb2p; HTB1; Htb1p; Histone H2B.1; Histone H2B.2; SPT12. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-Histone H2B.1 (Thr129) Recombinant Antibody, Cy5.5 Conjugated (BSM-61195R-CY5-5) validated for, and at what dilution?
BSM-61195R-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Histone H2B.1 (Thr129) Recombinant Antibody, Cy5.5 Conjugated (BSM-61195R-CY5-5) react with?
Tested reactivity: Human, Yeast.
How should BSM-61195R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Histone H2B.1 (Thr129) Recombinant Antibody, Cy5.5 Conjugated (BSM-61195R-CY5-5)?
KLH conjugated synthetic peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61195R-CY5-5)?
Cy5.5 on BSM-61195R-CY5-5: excitation / emission 675nm/694nm.