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ACSS2 Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61364r-cy5-100uL
Product Name ACSS2 Recombinant Antibody, Cy5 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ACSS2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55902
Swiss Prot Q9NR19
Synonyms ACSS2; ACSA; ACAS2; AceCS; MYH7B; Acetyl CoA synthetase.
Background Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids.Acetate is the preferred substrate.Can also utilize propionate with a much lower affinity,Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ACSS2 Recombinant Antibody, Cy5 Conjugated (bsm-61364R-Cy5) validated for, and at what dilution?

bsm-61364R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACSS2 Recombinant Antibody, Cy5 Conjugated (bsm-61364R-Cy5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.

How should bsm-61364R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACSS2 Recombinant Antibody, Cy5 Conjugated (bsm-61364R-Cy5)?

KLH conjugated synthetic peptide derived from human ACSS2

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61364R-Cy5)?

Cy5 on bsm-61364R-Cy5: excitation / emission 625,650nm/670nm.

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