VCP Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61451r-Cy5.5-100uL |
| Product Name | VCP Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VCP |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7415 |
| Swiss Prot | P55072 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 15S Mg(2+) ATPase p97 subunit; ALS14; ATPase p97; CDC48; IBMPFD; p97; TER ATPase; TERA; VCP; Yeast Cdc48p homolog. |
| Background | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is VCP Recombinant Antibody, Cy5.5 Conjugated (BSM-61451R-CY5-5) validated for, and at what dilution?
BSM-61451R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VCP Recombinant Antibody, Cy5.5 Conjugated (BSM-61451R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61451R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VCP Recombinant Antibody, Cy5.5 Conjugated (BSM-61451R-CY5-5)?
KLH conjugated synthetic peptide derived from human VCP
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61451R-CY5-5)?
Cy5.5 on BSM-61451R-CY5-5: excitation / emission 675nm/694nm.