ULK3 Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61618r-Cy5.5-100uL |
| Product Name | ULK3 Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ULK3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25989 |
| Swiss Prot | Q6PHR2 |
| Subcellular location | Nucleus |
| Synonyms | Serine/threonine-protein kinase ULK3; Ulk3; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3. |
| Background | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ULK3 Recombinant Antibody, Cy5.5 Conjugated (BSM-61618R-CY5-5) validated for, and at what dilution?
BSM-61618R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ULK3 Recombinant Antibody, Cy5.5 Conjugated (BSM-61618R-CY5-5) react with?
Tested reactivity: Human.
How should BSM-61618R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ULK3 Recombinant Antibody, Cy5.5 Conjugated (BSM-61618R-CY5-5)?
KLH conjugated synthetic peptide derived from human ULK3
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61618R-CY5-5)?
Cy5.5 on BSM-61618R-CY5-5: excitation / emission 675nm/694nm.