UBE3A Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61643r-Cy5.5-100uL |
| Product Name | UBE3A Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBE3A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7337 |
| Swiss Prot | Q05086 |
| Subcellular location | Nucleus |
| Synonyms | Ubiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A. |
| Background | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is UBE3A Recombinant Antibody, Cy5.5 Conjugated (BSM-61643R-CY5-5) validated for, and at what dilution?
BSM-61643R-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBE3A Recombinant Antibody, Cy5.5 Conjugated (BSM-61643R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61643R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBE3A Recombinant Antibody, Cy5.5 Conjugated (BSM-61643R-CY5-5)?
KLH conjugated synthetic peptide derived from human UBE3A
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61643R-CY5-5)?
Cy5.5 on BSM-61643R-CY5-5: excitation / emission 675nm/694nm.