Tropomyosin Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61923r-Cy5.5-100uL |
| Product Name | Tropomyosin Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tropomyosin 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7168 |
| Swiss Prot | P09493 |
| Subcellular location | Cytoplasm |
| Synonyms | CMH3; TMSA; CMD1Y; C15orf13; HTM-alpha. |
| Background | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Tropomyosin Recombinant Antibody, Cy5.5 Conjugated (BSM-61923R-CY5-5) validated for, and at what dilution?
BSM-61923R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tropomyosin Recombinant Antibody, Cy5.5 Conjugated (BSM-61923R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should BSM-61923R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tropomyosin Recombinant Antibody, Cy5.5 Conjugated (BSM-61923R-CY5-5)?
KLH conjugated synthetic peptide derived from human Tropomyosin 1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61923R-CY5-5)?
Cy5.5 on BSM-61923R-CY5-5: excitation / emission 675nm/694nm.