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ADAP/FYB Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61997r-cy5-100uL
Product Name ADAP/FYB Recombinant Antibody, Cy5 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FYN binding protein 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2533
Swiss Prot O15117
Subcellular location Cytoplasm, Nucleus
Synonyms ADAP; Fyb; FYN binding protein; p120/p130; SLAP130.
Background Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells.May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ADAP/FYB Recombinant Antibody, Cy5 Conjugated (bsm-61997R-Cy5) validated for, and at what dilution?

bsm-61997R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAP/FYB Recombinant Antibody, Cy5 Conjugated (bsm-61997R-Cy5) react with?

Tested reactivity: Human, Mouse.

How should bsm-61997R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADAP/FYB Recombinant Antibody, Cy5 Conjugated (bsm-61997R-Cy5)?

KLH conjugated synthetic peptide derived from human FYN binding protein 1

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61997R-Cy5)?

Cy5 on bsm-61997R-Cy5: excitation / emission 625,650nm/670nm.

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