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ALYREF Recombinant Antibody, Cy5.5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62100r-Cy5.5-100uL
Product Name ALYREF Recombinant Antibody, Cy5.5 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human THOC4
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10189
Swiss Prot Q86V81
Subcellular location Cytoplasm, Nucleus
Synonyms ALY; ALY/REF; BEF; REF; Tho4; thoc4.
Background Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export.Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ALYREF Recombinant Antibody, Cy5.5 Conjugated (BSM-62100R-CY5-5) validated for, and at what dilution?

BSM-62100R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ALYREF Recombinant Antibody, Cy5.5 Conjugated (BSM-62100R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat.

How should BSM-62100R-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ALYREF Recombinant Antibody, Cy5.5 Conjugated (BSM-62100R-CY5-5)?

KLH conjugated synthetic peptide derived from human THOC4

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-62100R-CY5-5)?

Cy5.5 on BSM-62100R-CY5-5: excitation / emission 675nm/694nm.

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