VPS11 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62133r-cy7-100uL |
| Product Name | VPS11 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS11 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55823 |
| Swiss Prot | Q9H270 |
| Subcellular location | Cytoplasm |
| Synonyms | END1; hVPS11; PEP5; PP3476; RNF108; vps11. |
| Background | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-200 |
Frequently asked questions
Which applications is VPS11 Recombinant Antibody, Cy7 Conjugated (bsm-62133R-Cy7) validated for, and at what dilution?
bsm-62133R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS11 Recombinant Antibody, Cy7 Conjugated (bsm-62133R-Cy7) react with?
Tested reactivity: Human, Mouse.
How should bsm-62133R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS11 Recombinant Antibody, Cy7 Conjugated (bsm-62133R-Cy7)?
KLH conjugated synthetic peptide derived from human VPS11
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-62133R-Cy7)?
Cy7 on bsm-62133R-Cy7: excitation / emission 743nm/767nm.