VPS18 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62138r-cy5-100uL |
| Product Name | VPS18 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS18 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57617 |
| Swiss Prot | Q9P253 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | hVPS18; PEP3; vps18. |
| Background | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is VPS18 Recombinant Antibody, Cy5 Conjugated (bsm-62138R-Cy5) validated for, and at what dilution?
bsm-62138R-Cy5 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS18 Recombinant Antibody, Cy5 Conjugated (bsm-62138R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62138R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS18 Recombinant Antibody, Cy5 Conjugated (bsm-62138R-Cy5)?
KLH conjugated synthetic peptide derived from human VPS18
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-62138R-Cy5)?
Cy5 on bsm-62138R-Cy5: excitation / emission 625,650nm/670nm.