VPS24 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62190r-cy3-100uL |
| Product Name | VPS24 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS24 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51652 |
| Swiss Prot | Q9Y3E7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | VPS24; CGI149; CHMP3; hVps24; NEDF. |
| Background | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-200 |
Frequently asked questions
Which applications is VPS24 Recombinant Antibody, Cy3 Conjugated (bsm-62190R-Cy3) validated for, and at what dilution?
bsm-62190R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS24 Recombinant Antibody, Cy3 Conjugated (bsm-62190R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62190R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS24 Recombinant Antibody, Cy3 Conjugated (bsm-62190R-Cy3)?
KLH conjugated synthetic peptide derived from human VPS24
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62190R-Cy3)?
Cy3 on bsm-62190R-Cy3: excitation / emission 512,550nm/570,615nm.