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ACAA2 Recombinant Antibody, Cy5.5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62464r-Cy5.5-100uL
Product Name ACAA2 Recombinant Antibody, Cy5.5 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ACAA2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10449
Swiss Prot P42765
Synonyms Acaa2; DSAEC.
Background In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ACAA2 Recombinant Antibody, Cy5.5 Conjugated (BSM-62464R-CY5-5) validated for, and at what dilution?

BSM-62464R-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACAA2 Recombinant Antibody, Cy5.5 Conjugated (BSM-62464R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat.

How should BSM-62464R-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACAA2 Recombinant Antibody, Cy5.5 Conjugated (BSM-62464R-CY5-5)?

KLH conjugated synthetic peptide derived from human ACAA2

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-62464R-CY5-5)?

Cy5.5 on BSM-62464R-CY5-5: excitation / emission 675nm/694nm.

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