UBE2L3 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62598r-cy3-100uL |
| Product Name | UBE2L3 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBE2L3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7332 |
| Swiss Prot | P68036 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | UBCE7; UbcH7; UbcM4; Ube2l3. |
| Background | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is UBE2L3 Recombinant Antibody, Cy3 Conjugated (bsm-62598R-Cy3) validated for, and at what dilution?
bsm-62598R-Cy3 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBE2L3 Recombinant Antibody, Cy3 Conjugated (bsm-62598R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62598R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBE2L3 Recombinant Antibody, Cy3 Conjugated (bsm-62598R-Cy3)?
KLH conjugated synthetic peptide derived from human UBE2L3
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62598R-Cy3)?
Cy3 on bsm-62598R-Cy3: excitation / emission 512,550nm/570,615nm.