Vimentin Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63101r-cy3-100uL |
| Product Name | Vimentin Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Vimentin |
| Clonality | Recombinant |
| Clone # | 4E11 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P08670 |
| Subcellular location | Cytoplasm |
| Synonyms | VIME_HUMAN; VIM; VIME_MOUSE; VIME_RAT; |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:50-200 |
| Flow-Cyt | Flow-Cyt=1:50-100 |
Frequently asked questions
Which applications is Vimentin Monoclonal Antibody, Cy3 Conjugated (bsm-63101R-Cy3) validated for, and at what dilution?
bsm-63101R-Cy3 is validated for WB (WB=1:500-2000); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vimentin Monoclonal Antibody, Cy3 Conjugated (bsm-63101R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63101R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Vimentin Monoclonal Antibody, Cy3 Conjugated (bsm-63101R-Cy3)?
A synthesized peptide derived from human Vimentin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63101R-Cy3)?
Cy3 on bsm-63101R-Cy3: excitation / emission 512,550nm/570,615nm.