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Acetyl-Histone H3(K9) Monoclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-63243r-cy3-100uL
Product Name Acetyl-Histone H3(K9) Monoclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human, Mouse
Specifications
Conjugation Cy3
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the acetylation site of K9
Clonality Recombinant
Clone # 6E6
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (ac-K10); ac-Histone H3; acetyl-Histone H3; H3K10Ac; ac-H3;
Application Dilution
WB =1:1000-2000
IF IF=1:50-200

Frequently asked questions

Which applications is Acetyl-Histone H3(K9) Monoclonal Antibody, Cy3 Conjugated (bsm-63243R-Cy3) validated for, and at what dilution?

bsm-63243R-Cy3 is validated for WB (WB=1:1000-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Acetyl-Histone H3(K9) Monoclonal Antibody, Cy3 Conjugated (bsm-63243R-Cy3) react with?

Tested reactivity: Human, Mouse.

How should bsm-63243R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Acetyl-Histone H3(K9) Monoclonal Antibody, Cy3 Conjugated (bsm-63243R-Cy3)?

A synthesized peptide derived from human Histone H3.1 around the acetylation site of K9

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63243R-Cy3)?

Cy3 on bsm-63243R-Cy3: excitation / emission 512,550nm/570,615nm.