Acetyl-Histone H3(K18) Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63269r-cy3-100uL |
| Product Name | Acetyl-Histone H3(K18) Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H3.1 around the acetylation site of K18 |
| Clonality | Recombinant |
| Clone # | 1F12 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3C1 | Histone H3 (acetyl-K19); H3K19Ac; ac-Histone H3; acetyl-Histone H3; ac-H3; H3C1 | Histone H3 (acetyl-Lys19); |
| Application Dilution | |
| WB | =1:1000-2000 |
| IF | IF=1:100-200 |
| Flow-Cyt | Flow-Cyt=1:50-100 |
Frequently asked questions
Which applications is Acetyl-Histone H3(K18) Recombinant Antibody, Cy3 Conjugated (bsm-63269R-Cy3) validated for, and at what dilution?
bsm-63269R-Cy3 is validated for WB (WB=1:1000-2000); IF (IF=1:100-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Acetyl-Histone H3(K18) Recombinant Antibody, Cy3 Conjugated (bsm-63269R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63269R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Acetyl-Histone H3(K18) Recombinant Antibody, Cy3 Conjugated (bsm-63269R-Cy3)?
A synthesized peptide derived from human Histone H3.1 around the acetylation site of K18
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63269R-Cy3)?
Cy3 on bsm-63269R-Cy3: excitation / emission 512,550nm/570,615nm.