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CRISPR, Gene Editing & DNA-Repair Antibodies

CRISPR efficiency is only part of the result. Evaluate DNA-damage signaling, repair-pathway activation, and editing outcomes with antibodies targeting Cas9, RAD51, BRCA2, DNA-PK, γH2AX, ATM, ATR, and other key genome-stability proteins.



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Catalog #1: bsm-61460r-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61460r-rbitc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61460r-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-61460r-pe-cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-61460r-bf750

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61460r-pe

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-61460r-bf594

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61460r-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-61460r-bf680

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61460r-bf647

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61460r-pe-cy3

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy3

Catalog #1: bsm-61460r-pe-cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-61460r-fitc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-61460r-cy5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-61460r-cy3

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-61460r-apc-cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5

Catalog #1: bsm-61460r-apc-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-61460r-bf488

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61460r-bf555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61460r-bf405

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61460r-apc-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-61460r-apc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-61460r-bf350

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

25 ug total protein per lane of various lysates (see on figure) probed with Ku70 \/ XRCC6 monoclonal antibody, unconjugated (bsm-61460R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61460r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: Jurkat cells lysates; Probed with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells with Ku70 (6D1) Monoclonal Antibody (bsm-52970R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human breast cancer tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Ku 70 (6D1) Monoclonal Antibody (bsm-52970R) at 1:30, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52970R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60941r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52960r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-63055r-percp-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-63055r-rbitc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-63056r-percp-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-63056r-rbitc

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-63055r-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-63055r-percp

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-63055r-pe-cy7

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-63055r-pe

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-63055r-pe-cy5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-63055r-pe-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-63055r-bf594

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-63055r-bf750

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-63055r-fitc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-63055r-bf555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-63055r-cy3

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-63055r-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-63055r-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-63055r-bf488

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-63055r-cy5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-63055r-bf680

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

25 ug total protein per lane of various lysates (see on figure) probed with ATM monoclonal antibody, unconjugated (bsm-63055R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATM Monoclonal Antibody, Unconjugated (bsm-63055R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATM Monoclonal Antibody, Unconjugated (bsm-63055R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The NCCIT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ATM antibody (bsm-63055R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-63055r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated