Phospho-BRCA1 (Ser1466) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12887R-Biotin-100uL |
| Product Name | Phospho-BRCA1 (Ser1466) Polyclonal Antibody, Biotin Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Ser1466 |
| Modification Site | Ser1466 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 672 |
| Synonyms | BRCA1 phospho S1466; BRCA1 phospho Ser1466; p-BRCA1 S1466; p-BRCA1 Ser1466; p-BRCA1 phospho S1466; BRCA1Ser1423; BRCA 1; BRCA1; BRCA1/BRCA2 containing complex subunit 1; BRCA1/BRCA2-containing complex, subunit 1; BRCA1_HUMAN; BRCAI; BRAC 1; BRCA 1; BRCC 1; BRCC1; Breast Cancer 1; Breast Cancer 1 Early Onset; Breast cancer type 1 susceptibility protein; Breast and ovarian cancer susceptibility protein 1; Breast Ovarian Cancer Susceptibility; IRIS; Papillary Serous Carcinoma Of The Peritoneum; PSCP; RING finger protein 53; BROVCA1; IRIS; PNCA4; PPP1R53; Protein phosphatase 1 regulatory subunit 53; RNF53; BAP1. |
| Background | This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]. |
| Application Dilution | |
| IHC-P | 1:100-500 |
| IHC-F | |
Frequently asked questions
Which applications is Phospho-BRCA1 (Ser1466) Polyclonal Antibody, Biotin Conjugated (bs-12887R-Biotin) validated for, and at what dilution?
bs-12887R-Biotin is validated for IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-BRCA1 (Ser1466) Polyclonal Antibody, Biotin Conjugated (bs-12887R-Biotin) react with?
Tested reactivity: Human.
How should bs-12887R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-BRCA1 (Ser1466) Polyclonal Antibody, Biotin Conjugated (bs-12887R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Ser1466