phospho-FANCD2 (Ser1404) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13141R-APC-Cy7-100uL |
| Product Name | phospho-FANCD2 (Ser1404) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FANCD2 around the phosphorylation site of Ser1404 |
| Modification Site | Ser1404 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2177 |
| Subcellular location | Nucleus |
| Synonyms | FANCD2 phospho S1404; FANCD2 phospho Ser1404; p-FANCD2 phospho S1404; FA 4; FA D2; FA4; FAC D2; FACD 2; FACD; FACD2; FAD; FAD2; FANC D2; FANCD 2; FANCD; Protein FACD2; Type 4 Fanconi pancytopenia; FACD2_HUMAN; Fanconi anemia group D2 protein; Protein FACD2. |
| Background | Fanconi Anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANC D2, targeting nuclear foci containing the BRCA 1 protein. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-FANCD2 (Ser1404) Polyclonal Antibody, APC-Cy7 Conjugated (bs-13141R-APC-Cy7) validated for, and at what dilution?
bs-13141R-APC-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-FANCD2 (Ser1404) Polyclonal Antibody, APC-Cy7 Conjugated (bs-13141R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.
How should bs-13141R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-FANCD2 (Ser1404) Polyclonal Antibody, APC-Cy7 Conjugated (bs-13141R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human FANCD2 around the phosphorylation site of Ser1404
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-13141R-APC-Cy7)?
APC-Cy7 on bs-13141R-APC-Cy7: excitation / emission 650nm/780nm.