XRCC5/Ku80 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1358R-100uL |
| Product Name | XRCC5/Ku80 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF
IHC-P
|
| Reactivity | Others |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ku-80 |
| Immunogen Range | 301-450/732 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7520 |
| Subcellular location | Nucleus |
| Synonyms | XRCC5; Ku80; TLAA; CTC85; Ku86; CTCBF; G22P2; KARP1; FLJ39089; KARP-1; NFIV; KU80; KUB2; EC 3.6.1. |
| Background | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is XRCC5/Ku80 Polyclonal Antibody (bs-1358R) validated for, and at what dilution?
bs-1358R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1358R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise XRCC5/Ku80 Polyclonal Antibody (bs-1358R)?
KLH conjugated synthetic peptide derived from human Ku-80 Immunogen range: 301-450/732.