Phospho-CHEK2 (Thr26) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13909R-Cy3-100uL |
| Product Name | Phospho-CHEK2 (Thr26) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr26 |
| Modification Site | Thr26 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11200 |
| Swiss Prot | O96017 |
| Synonyms | Chk2 (phospho T26); p-Chk2 (phospho T26); bA444G7; CHK2 checkpoint homolog; CHK2_HUMAN; Serine/threonine-protein kinase Chk2; CDS 1; CDS1; Checkpoint kinase 2; Checkpoint like protein CHK2; Chek 2; Chek2; Chk 2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog; HuCds 1; HuCds1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2. |
| Background | Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Phospho-CHEK2 (Thr26) Polyclonal Antibody, Cy3 Conjugated (bs-13909R-Cy3) validated for, and at what dilution?
bs-13909R-Cy3 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CHEK2 (Thr26) Polyclonal Antibody, Cy3 Conjugated (bs-13909R-Cy3) react with?
Tested reactivity: Human.
How should bs-13909R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CHEK2 (Thr26) Polyclonal Antibody, Cy3 Conjugated (bs-13909R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr26
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13909R-Cy3)?
Cy3 on bs-13909R-Cy3: excitation / emission 512,550nm/570,615nm.