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CHK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1681R-BF594-100uL
Product Name CHK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHK1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1111
Synonyms Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog S. pombe; CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog S. pombe; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A S. cerevisiae; C85740; CHK1_HUMAN.
Background The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].
Application Dilution
WB 1:500-2000
IF

Frequently asked questions

Which applications is CHK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1681R-BF594) validated for, and at what dilution?

bs-1681R-BF594 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1681R-BF594) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1681R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1681R-BF594)?

KLH conjugated synthetic peptide derived from human CHK1

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-1681R-BF594)?

AbBy Fluor-594 on bs-1681R-BF594: excitation / emission 590nm/617nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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