Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

Rad51 Polyclonal Antibody

Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.
Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.
HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4\u00b0C, DAB staining.
Tissue\/cell: FHC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: FHC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: H9C2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: H9C2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Rad51 Polyclonal Antibody, conjugated (bs-20297R-AF555) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Rad51 \r\n Antibody(bs-20297R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Rad51 \r\n Antibody(bs-20297R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Rad51) polyclonal Antibody, Unconjugated (bs-20297R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\r\n\r\n
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Rad51) polyclonal Antibody, Unconjugated (bs-20297R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\r\n\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-20297R-100uL
Product Name Rad51 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Rad51
Immunogen Range 81-180/339
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5888
Swiss Prot Q06609
Subcellular location Cytoplasm, Nucleus
Synonyms RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930; DNA repair protein RAD51 homolog 1; RAD51 homolog A; RAD51
Background Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Rad51 Polyclonal Antibody (bs-20297R) validated for, and at what dilution?

bs-20297R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Rad51 Polyclonal Antibody (bs-20297R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.

How should bs-20297R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Rad51 Polyclonal Antibody (bs-20297R)?

KLH conjugated synthetic peptide derived from human Rad51 Immunogen range: 81-180/339.

Search our catalog of hundreds of thousands of products

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

View product details and validation

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart