Phospho-Mre11 (Ser676) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3293R-100uL |
| Product Name | Phospho-Mre11 (Ser676) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676 |
| Modification Site | Ser676 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4361 |
| Subcellular location | Nucleus |
| Synonyms | Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 meiotic recombination 11 homolog A; MRE11a; MRE11b; AT like disease; Ataxia telangiectasia disorder like; Ataxia-telangiectasia disorder-like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11. HNGS1; meiotic recombination S. cerevisiae 11 homolog A; meiotic recombination 11 homolog A S. cerevisiae; meiotic recombination 11 homolog A; MmMRE11A.MRE11_RAT |
| Background | Mre11 is a component of the MRN complex, which plays a critical role in double-strand break repair (DSB), DNA recombination, maintenance of telomere integrity, and meiosis. Mre11 has both single-strand endonuclease and double strand-specific 3'-5' exonuclease activities. Rad50, another component of the MRN complex, is postulated to bind to DNA ends and hold them in close proximity, thus facilitating searches for regions of sequence homology, and also possibly stimulating the activity of DNA ligases and/or restricting Mre11 activity. Mutations in Mre11 may cause breast cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Mre11 (Ser676) Polyclonal Antibody (bs-3293R) validated for, and at what dilution?
bs-3293R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Mre11 (Ser676) Polyclonal Antibody (bs-3293R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog.
How should bs-3293R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Mre11 (Ser676) Polyclonal Antibody (bs-3293R)?
KLH conjugated synthetic phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676