PARP1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55164r-bf488-100uL |
| Product Name | PARP1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | Recombinant human PARP1 |
| Immunogen Range | 81-390/1014 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 142 |
| Swiss Prot | P09874 |
| Subcellular location | Nucleus |
| Synonyms | ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PARS; PPOL; Poly-PARP; pADPRT-1; 5830444G22Rik; Adprp; msPARP; sPARP-1; PARP1_HUMAN; PARP1; ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1); DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1 (ADPRT 1); Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; 2.4.2.30; PARP1_MOUSE; Poly[ADP-ribose] synthase 1 (msPARP); PARP1_RAT; poly(ADP-ribose) polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); poly (ADP-ribose) polymerase family, member 1 |
| Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is PARP1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-55164R-BF488) validated for, and at what dilution?
bs-55164R-BF488 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PARP1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-55164R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should bs-55164R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PARP1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-55164R-BF488)?
Recombinant human PARP1 Immunogen range: 81-390/1014.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-55164R-BF488)?
AbBy Fluor-488 on bs-55164R-BF488: excitation / emission 499nm/519nm.