PARP1 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55164r-pe-cy55-100uL |
| Product Name | PARP1 Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant human PARP1 |
| Immunogen Range | 81-390/1014 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 142 |
| Swiss Prot | P09874 |
| Subcellular location | Nucleus |
| Synonyms | ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PARS; PPOL; Poly-PARP; pADPRT-1; 5830444G22Rik; Adprp; msPARP; sPARP-1; PARP1_HUMAN; PARP1; ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1); DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1 (ADPRT 1); Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; 2.4.2.30; PARP1_MOUSE; Poly[ADP-ribose] synthase 1 (msPARP); PARP1_RAT; poly(ADP-ribose) polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); poly (ADP-ribose) polymerase family, member 1 |
| Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
| Application Dilution | |
| WB | 1:500-2000 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is PARP1 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-55164R-PE-CY5-5) validated for, and at what dilution?
BS-55164R-PE-CY5-5 is validated for WB (1:500-2000); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PARP1 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-55164R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should BS-55164R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PARP1 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-55164R-PE-CY5-5)?
Recombinant human PARP1 Immunogen range: 81-390/1014.
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-55164R-PE-CY5-5)?
PE-Cy5.5 on BS-55164R-PE-CY5-5: excitation / emission 488nm/694nm.