ATRIP (Ser239) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70024r-100uL |
| Product Name | ATRIP (Ser239) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~86 kDa ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32 kDa. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Bovine, Sheep, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser239 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 84126 |
| Swiss Prot | Q8WXE1 |
| Synonyms | AGS1 antibody, ATIP antibody, ATM and Rad3 related interacting protein antibody, ATM and Rad3-related-interacting protein antibody, ATR interacting protein antibody, ATR-interacting protein antibody, Atrip antibody, ATRIP_HUMAN antibody, DKFZp762J2115 antibody, FLJ12343 antibody, MGC20625 antibody, MGC21482 antibody, MGC26740 antibody |
| Background | ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser-239 (Venere et al, 2007). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ATRIP (Ser239) Antibody (bs-70024R) validated for, and at what dilution?
bs-70024R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATRIP (Ser239) Antibody (bs-70024R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Bovine, Sheep, Non-Human Primate.
How should bs-70024R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise ATRIP (Ser239) Antibody (bs-70024R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH).