RAD51 (1C9) Monoclonal Antibody

$Lane 1: Jurkat Cell lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: C6 Cell lysates; Lane 4: PC-12 Cell lysates; Lane 5: Hela Cell lysates; probed with RAD51 (1C9) Monoclonal Antibody, unconjugated (bsm-51402M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat Cell lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: C6 Cell lysates; Lane 4: PC-12 Cell lysates; Lane 5: Hela Cell lysates; probed with RAD51 (1C9) Monoclonal Antibody, unconjugated (bsm-51402M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with RAD5 Monoclonal Antibody, Unconjugated (bsm-51402M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with RAD5 Monoclonal Antibody, Unconjugated (bsm-51402M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bsm-51402M
Product Name	RAD51 (1C9) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombination human RAD51 protein
Clonality	Monoclonal
Clone #	3G10
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5888
Swiss Prot	Q06609
Subcellular location	Cytoplasm, Nucleus
Synonyms	BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51A; RECA; RGD1563603; RAD51_HUMAN; RAD51; RAD51 homolog A; RAD51_MOUSE; RAD51 recombinase; RAD51 (S. cerevisiae) homolog (E coli RecA homolog); RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog (S. cerevisiae); BRCA1/BRCA2-containing complex, subunit 5
Background	Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Application Dilution
WB	1:300-5000