Phospho-H2AX (Ser139) Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52163r-biotin-100uL |
| Product Name | Phospho-H2AX (Ser139) Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Ser139:QA(p-S)QE. |
| Modification Site | S139 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3014 |
| Swiss Prot | P16104 |
| Synonyms | H2A histone family member X; Histone H2A.x; H2afx; H2a.x; H2ax; Hist5-2ax; H2A.X; H2A/X; H2AX; H2AFX; H2AX_HUMAN. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-H2AX (Ser139) Recombinant Antibody, Biotin Conjugated (bsm-52163R-Biotin) validated for, and at what dilution?
bsm-52163R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-H2AX (Ser139) Recombinant Antibody, Biotin Conjugated (bsm-52163R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52163R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-H2AX (Ser139) Recombinant Antibody, Biotin Conjugated (bsm-52163R-Biotin)?
KLH conjugated Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Ser139:QA(p-S)QE.