Chk2 (7A4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52522R-100uL |
| Product Name | Chk2 (7A4) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human Chk2 between 1-200 amino acids |
| Clonality | Monoclonal |
| Clone # | 7A4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 11200 |
| Swiss Prot | O96017 |
| Synonyms | Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1, CHEK2 |
| Background | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Chk2 (7A4) Monoclonal Antibody (bsm-52522R) validated for, and at what dilution?
bsm-52522R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Chk2 (7A4) Monoclonal Antibody (bsm-52522R) react with?
Tested reactivity: Human.
How should bsm-52522R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Chk2 (7A4) Monoclonal Antibody (bsm-52522R)?
Human Chk2 between 1-200 amino acids