Phospho-RPA32/RPA2 (T21) (10A4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52578R-100uL |
| Product Name | Phospho-RPA32/RPA2 (T21) (10A4) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide surrounding Thr21 of human RPA32/RPA2 |
| Modification Site | T21 |
| Clonality | Monoclonal |
| Clone # | 10A4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Swiss Prot | P15927 |
| Synonyms | Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit, RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34 |
| Background | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-RPA32/RPA2 (T21) (10A4) Monoclonal Antibody (bsm-52578R) validated for, and at what dilution?
bsm-52578R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-RPA32/RPA2 (T21) (10A4) Monoclonal Antibody (bsm-52578R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52578R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-RPA32/RPA2 (T21) (10A4) Monoclonal Antibody (bsm-52578R)?
Synthetic phospho-peptide surrounding Thr21 of human RPA32/RPA2